Alpha¹ by KaimenConfidential

Kaimen Rigor review

Slug enhances invasion ability of pancreatic cancer cells through upregulation of matrix metalloproteinase-9 and actin cytoskeleton remodeling

Zhang K, Chen D, Jiao X, Zhang S, Liu X, Cao J, Wu L, Wang D.

DOI: 10.1038/labinvest.2010.201
Record issued: 2026-08-06
Engine: 7.15.0
Exported: 2026-09-20

Prepared by Alpha1. This document is confidential: it is intended for the recipient it was shared with and must not be redistributed. The live record at alpha1science.com/verify/975285ad-8c12-496d-b45f-03bf30db2955 is authoritative.

Kaimen Rigor review

Kaimen Rigor reviewed

1 / 5

Critical — a demonstrable failure

Mixed Methods

Critical

High-priority

How this rating was calculated

Started at 5★ — deductions

IntegrityIntegrity concern ×7−3.5★
ClaimsOverstated claim ×3−1.5★
StatisticsImpossible or misreported statistic−1★
StatisticsStatistic did not reproduce−0.5★
ReportingBiological variables not met−0.5★
ReportingData & code availability not met−0.5★
CopyeditManuscript needs significant editing−0.5★
ReportingStudy design partially met−0.25★
ReportingEthical approvals partially met−0.25★
ReportingKey resources partially met−0.25★
ReportingStatistical analysis partially met−0.25★
ReportingReporting transparency partially met−0.25★

A demonstrable critical failure caps the rating at the minimum, regardless of the deductions above.

Model-reported confidence: moderateReviewer agreement: lowEngine: 7.15.0Source: full text

Not verified in this report

No data or code availability links were detected to verify.

Biggest issues1 demonstrable

01Significance claim does not survive recomputationdemonstrable
criticalOverreachrecomputedFull detail
Chi-square test for association between E-cadherin expression and nodal metastasis (Table 1)
02Reported statistic does not recompute
highArithmeticrecomputedFull detail
Chi-square test for association between Slug expression and nodal metastasis (Table 1)
03Biological variables not reported
highDisclosurereviewer-assessedFull detail
Biological variables are inadequately reported: human patient demographics (age, sex) are absent, animal housing conditions are vague, and single-sex animal use is not justified.
“Female C57BL/6 mice at 6–8 weeks old were obtained from Qingdao Medical college, Qingdao University”
Methods
04Data and code not shared
highDisclosurereviewer-assessedFull detail
No data availability statement or data/code sharing is provided. The paper does not mention any repository deposit, accession numbers, or code repository.
05Internal contradictions in the reported numbers
mediumArithmeticreviewer-assessedFull detail
In Table 2 the Slug column totals (18+17=35 for 'Positive' and 7+18=25 for 'Negative') directly contradict the stated group sizes (Positive n=41, Negative n=19) given in the same table header; the reported P=0.0034 for the Slug-MMP-9 correlation does not reproduce from either reading of the displayed counts.
Slug | Positive ( n =41) | Negative ( n =19) | | MMP-9 | Positive ( n =25) | 18 | 7 | | Negative ( n =35) | 17 | 18
Table 2reviewer’s wording
06Internal contradictions in the reported numbers
mediumArithmeticreviewer-assessedFull detail
In Table 2, the Slug-positive column sums to 35 and the Slug-negative column sums to 25, but the table states n=41 and n=19, respectively. This internal inconsistency undermines the reported correlation p-values.
“Positive ( n =41) | Negative ( n =19) | | | MMP-9 | | | 0.0034 | | Positive ( n =25) | 18 | 7 | | | Negative ( n =35) | 17 | 18”
Table 2

3 further findings of this severity or below — every one is in the sections below, filed under its error type.

Findings by category

201ArithmeticNumerical inconsistencies 302OverreachOverstated conclusions 203AuthenticityData authenticity concerns 704DisclosureReporting gaps 05AvailabilityNone found · partly checked 106CopyeditCopyediting

This Kaimen Rigor review uses Kaimen Rigor reviewers trained on a curated corpus of high-fidelity and retracted papers, with expert supervision and curation. It can still make mistakes; verify each finding against the source before relying on it.

The paper has a clear scientific premise and adequate controls for experiments, but lacks many fundamental rigor elements: no randomization, blinding, power analysis, or independent replication; human cohort demographics are missing; key resources lack catalog numbers and cell line authentication; statistical reporting contains inconsistencies in Table 2 that undermine the reported p-values; no data or code availability; and several conclusions are overstated relative to the evidence.

All eight dimensions were applicable. The three independent reviewer runs agreed on the overall pattern but diverged on the severity of study design, ethical approvals, key resources, statistical analysis, and reporting transparency; the synthesized statuses were determined by applying the scoring rules to the checklist evidence, which sometimes corrected overly lenient ratings.

Numerical inconsistencies

2 findings · worst high

Values that contradict each other or are impossible for the stated sample: recomputed p-values and test statistics, GRIM/GRIMMER checks on summary numbers, percentages against their own counts, totals against their parts, and estimates against their own confidence intervals.

Reported statistics do not recomputeRecomputed
Internal contradictions in the reported numbersAssessed

Recomputed 7 tests: 5 consistent, 2 inconsistent (1 change significance at p<.05); 7 via agent-written checks.

Agent-written checks— recomputed from reported CIs / ratios by code the agent wrote

INCONSISTENTreported p < .010 · recomputed p = .018Reviewers 1, 3
Chi-square test for association between Slug expression and nodal metastasis (Table 1)
How we recomputed it: pChi2x2(19,15,22,4)
CONSISTENTreported p < .010 · recomputed p = .006Reviewers 1, 3
Chi-square test for association between MMP-9 expression and nodal metastasis (Table 1)
How we recomputed it: pChi2x2(9,25,16,10)
CONSISTENTreported p > .050 · recomputed p = .109Reviewer 1
Chi-square test for correlation between Slug and E-cadherin expression (Table 2)
How we recomputed it: pChi2x2(11,13,24,12)
CONSISTENTreported p = .057 · recomputed p = .109Reviewer 3
Slug vs E-cadherin association, Table 2, printed cell counts
How we recomputed it: pChi2x2(11,13,24,12)
CONSISTENTreported p < .050 · recomputed p = <.001Reviewer 3
MMP-9 expression vs nervous invasion, Table 1
How we recomputed it: pChi2x2(7,25,18,10)
CONSISTENTreported p > .050 · recomputed p = .630Reviewer 3
Slug expression vs nervous invasion, Table 1
How we recomputed it: pChi2x2(21,11,20,8)

mediuminternal contradiction
In Table 2 the Slug column totals (18+17=35 for 'Positive' and 7+18=25 for 'Negative') directly contradict the stated group sizes (Positive n=41, Negative n=19) given in the same table header; the reported P=0.0034 for the Slug-MMP-9 correlation does not reproduce from either reading of the displayed counts.
Slug | Positive ( n =41) | Negative ( n =19) | | MMP-9 | Positive ( n =25) | 18 | 7 | | Negative ( n =35) | 17 | 18
Table 2reviewer’s wording
mediuminternal contradiction
In Table 2, the Slug-positive column sums to 35 and the Slug-negative column sums to 25, but the table states n=41 and n=19, respectively. This internal inconsistency undermines the reported correlation p-values.
“Positive ( n =41) | Negative ( n =19) | | | MMP-9 | | | 0.0034 | | Positive ( n =25) | 18 | 7 | | | Negative ( n =35) | 17 | 18”
Table 2
lowinternal contradiction
The Animal Studies section describes 'Female C57BL/6 mice' but subsequently refers to them as 'nude mice'; while C57BL/6 nude mice exist, the nomenclature is inconsistent and the strain background is ambiguous.
Female C57BL/6 mice at 6–8 weeks old were obtained from Qingdao Medical college, Qingdao University for tumor implantation. All animals were maintained in a sterile environment and cared for within the laboratory animal regulations of the Ministry of Science and Technology of the People's Republic of China. Full details of the study approval by the ethics committee at the affiliated hospital of medical college, Qingdao University. We inoculated the nude mice with cell samples as described previously.
Methodsreviewer’s wording
lowinternal contradiction
Table 3's title states n=10 while the groups are labeled n=12 and all cell counts (e.g., 8+4=12 for PANC-1 hepatic) sum to 12, so the (n=10) header appears to be a typo.
“Table 3 Slug promoted in vivo tumor hepatic metastasis and lymph node invasion ( n =10) Groups ( n =12)”
Table 3
lowinternal contradiction
Table 3 caption says n=10 while the table header and Results text indicate n=12 per group.
“Table 3 Slug promoted in vivo tumor hepatic metastasis and lymph node invasion ( n =10) Groups ( n =12)”
Table 3

Overstated conclusions

3 findings · worst critical

Conclusions that reach past what the paper's own results support — including a significance claim that no longer holds when the statistic is recomputed, and efficacy resting on an unvalidated surrogate endpoint.

Significance claim flips when recomputedRecomputed
Conclusions overstated beyond the evidenceAssessed
Conclusions only partially backed by the presented evidenceAssessed

Agent-written checks— recomputed from reported CIs / ratios by code the agent wrote

SIGNIFICANCE OVERSTATEDINCONSISTENTreported p < .010 · recomputed p = .071Reviewers 1, 3
Chi-square test for association between E-cadherin expression and nodal metastasis (Table 1)
Reported as statistically significant, but recomputing from the paper’s own numbers gives p ≥ 0.05 — the result may not be significant as claimed.
How we recomputed it: pChi2x2(17,17,7,19)

12 major claims checked against the paper's own evidence: 3 not fully backed by the presented evidence (unsupported or overstated).

overstatedReviewer 1
Slug transports MMP-9 to the anterior extremity of filopodia via F-actin networks.
The claim overstates the evidence; the study shows co-localization of MMP-9 and F-actin in filopodia, but does not demonstrate direct transport or a causal mechanism for transport.
Evidence: Figure 5: double immunofluorescence shows MMP-9 and F-actin co-localize in filopodia of Slug-transfected cells.
MMP-9 and F-actin are primarily located in the anterior extremity of the filopodia... suggesting Slug might transport MMP-9 via F-actin networks
Discussionreviewer’s wording
overstatedReviewer 2
Slug may serve as a marker for predicting malignancy of pancreatic cancer after surgery.
This is a correlative IHC finding in 60 patients with no prospective validation, sensitivity/specificity analysis, or multivariate adjustment, so the predictive-marker claim reaches beyond the evidence.
Evidence: Only the IHC association with nodal metastasis in Table 1; no predictive performance analysis.
“Slug may serve as a marker for the prediction of the malignancy of pancreatic cancer after operation.”
Discussion ¶2
overstatedReviewer 3
The present study is the first to explore the relationship between Slug and MMP-9 in pancreatic cancer.
The paper provides evidence for an association and mechanism but does not provide any literature-search basis to substantiate the 'for the first time' priority claim.
Evidence: No systematic literature review or novelty declaration is provided.
“The present study for the first time has explored the relationship between Slug and MMP-9 in pancreatic cancer and clarified the novel mechanism.”
Discussion
partialReviewer 2
There is a significant correlation between Slug and MMP-9 expression in pancreatic cancer tissue.
The paper reports P=0.0034 for the Slug-MMP-9 correlation, but the supporting Table 2 has cell counts that do not sum to the stated group sizes and the p-value does not reproduce from the displayed numbers, so the correlation is not reliably substantiated by the presented data.
Evidence: Table 2 Slug-MMP-9 cross-tabulation with P=0.0034; Slug column sums (35/25) contradict stated n (41/19).
“Statistically significant correlations were found between Slug and MMP-9 expression ( P <0.05).”
Table 2
partialReviewer 3
A significant correlation exists between Slug and MMP-9 expression.
The paper reports p=0.0034, but Table 2's cell counts do not sum to the stated group sizes, so the correlation is not verifiable from the printed data.
Evidence: Table 2 reports 0.0034 for the Slug–MMP-9 correlation.
“Statistically significant correlations were found between Slug and MMP-9 expression ( P <0.05).”
Results
partialReviewer 3
There is no significant correlation between Slug and E-cadherin expression.
The reported p=0.057 supports the conclusion directionally, but the same Table 2 inconsistency prevents full verification.
Evidence: Table 2 reports 0.057 for the Slug–E-cadherin correlation.
“no significant correlation was observed between Slug and E-cadherin expression”
Abstract
supportedReviewer 1
Slug expression is significantly associated with lymph node metastases in pancreatic cancer patients.
The claim is supported by Table 1 data showing a significant chi-square test (P<0.01) for nodal metastasis vs. Slug expression.
Evidence: Table 1: Nodal metastasis positive group: Slug+ 22, Slug- 4; negative group: Slug+ 19, Slug- 15; χ²=5.49, P<0.01.
Nodal metastasis... P <0.01
Table 1reviewer’s wording
supportedReviewer 1
Slug expression is significantly correlated with MMP-9 expression but not with E-cadherin expression in pancreatic cancer.
The claim is supported by Table 2 which shows a significant association between Slug and MMP-9 (P=0.0034) and no significant association between Slug and E-cadherin (P=0.057).
Evidence: Table 2: MMP-9 vs. Slug: χ² test P=0.0034; E-cadherin vs. Slug: P=0.057.
Correlation between Slug expression and E-cadherin or MMP-9 expression... P 0.0034
Table 2reviewer’s wording
supportedReviewer 1
Slug overexpression upregulates MMP-9 and promotes invasion and metastasis in PANC-1 cells.
The claim is supported by multiple in vitro assays (RT-PCR, western blot, ELISA, zymography) showing increased MMP-9 expression and activity, and by invasion assays and in vivo xenograft showing increased metastasis.
Evidence: Figures 2, 6, 7; Table 3: increased hepatic and lung metastasis in Slug-transfected group.
Slug overexpression upregulated MMP-9 at the mRNA and protein level... overexpression of Slug enhanced invasion in PANC-1 cells... Slug-transfected cells exhibited a highly invasive behavior
Resultsreviewer’s wording
supportedReviewer 1
Slug promotes F-actin polymerization and filopodia formation in PANC-1 cells.
The claim is supported by FCM showing increased intracellular F-actin and confocal microscopy showing filopodia formation and F-actin redistribution.
Evidence: Figure 4: FCM shows 2.1-fold increase in F-actin; confocal microscopy shows F-actin redistribution in Slug-transfected cells.
the intracellular F-actin in Slug-transfected clones increased 2.1-fold... F-actin was polymerized in Slug-transfected clones
Resultsreviewer’s wording
supportedReviewer 2
Slug, E-cadherin, and MMP-9 expression in pancreatic cancer tissue is significantly associated with lymph node metastases.
Table 1 reports significant chi-square associations for all three markers with nodal metastasis (P<0.01), consistent with the claim.
Evidence: Table 1 Nodal metastasis rows: Slug chi2=5.49 P<0.01; MMP-9 chi2=5.24 P<0.01; E-cadherin chi2=4.86 P<0.01.
“Slug, MMP-9, and E-cadherin expression was significantly associated with nodal metastases ( P <0.01, respectively”
Results
supportedReviewer 2
Slug transfection significantly increases invasion and metastasis of PANC-1 cells in vitro and in an orthotopic mouse model, and upregulates and activates MMP-9.
In vitro invasion counts (68.74±7.34 vs 117.16±8.45), zymography showing active 82-kDa MMP-9, and in vivo metastasis counts (10 liver, 6 lung in Slug-transfected vs fewer in controls) support the claim.
Evidence: Cell Invasion Assays results; Gelatin Zymography; Table 3 in vivo metastasis counts.
“overexpression of Slug enhanced invasion in PANC-1 cells. The difference was statistically significant”
Results

Data authenticity concerns

2 findings · worst low

An adversarial read for patterns associated with data that may not be genuine: results that look too clean, implausibly large effects, duplicated data or images, and methods that do not match the results reported.

Methods and results do not matchAssessed
Other integrity concernAssessed

7 integrity concerns flagged (0 high).

lowother
Several self-reported chi-square statistics in Table 1, when converted with df=1, give p-values around 0.02-0.07 (e.g., Slug nodal chi-square=5.49 -> p~0.019, E-cadherin nodal chi-square=4.86 -> p~0.027), which are below 0.05 but not below the reported threshold of 0.01, suggesting the 'P<0.01' labels may be slightly overstated.
Nodal metastasis | Negative ( n =34) | 19 | 15 | | Positive ( n =26) | 22 | 4 | | ... | 5.49 | <0.01
Table 1reviewer’s wording
lowmethod result mismatch
The reported p=0.0034 for the Slug–MMP-9 correlation is not reproduced by Pearson chi-square on the printed table cell counts (approximate p=0.07), although the table's internal inconsistency makes the exact cause unclear.
“Statistically significant correlations were found between Slug and MMP-9 expression ( P <0.05).”
Table 2

Reporting gaps

7 findings · worst high

Required detail the manuscript never states — study design, biological variables, ethics approval and consent, key resources, statistical reporting, data and code availability, and overall transparency.

Biological variables not reportedAssessed
Data and code not sharedAssessed
Statistical reporting gaps (tests, assumptions, effect sizes)Assessed
Ethics/consent reporting incompleteAssessed
Key resources under-identified (antibodies, cell lines, RRIDs)Assessed
Reporting/transparency gapsAssessed
Study-design details incomplete (controls, blinding, power)Assessed

Eight Dimensions1 pass · 5 warn · 2 fail

Prior research on Slug, EMT, E-cadherin, and MMP-9 in pancreatic cancer is cited, including studies that found no relationship between Slug and E-cadherin. The rationale for investigating Slug's role in MMP-9 upregulation is clearly stated. Limitations of prior work are acknowledged (e.g., dependency on tissue/cell type), and the study aims to address these gaps.

Sub-criteria

prior work citedADEQUATE

premise rationaleADEQUATE

limitations addressedADEQUATE

Per reviewer (3) — the votes behind the verdict

PASSReviewer 1· 90% conf

The paper establishes a clear scientific foundation by citing relevant prior work, identifying gaps in knowledge, and presenting a logical rationale for the study.

Evidence

“A recent study demonstrated that no relationship exists between Slug and E-cadherin in pancreatic cancer.”

Introduction ¶1

“In this study, we aimed to examine the expression of Slug, E-cadherin, and MMP-9 in pancreatic cancer and to obtain further insight into the question of whether Slug promotes E-cadherin or MMP-9-induced EMT in pancreatic cancer.”

Introduction ¶5

“the relationship between Slug transcription, Snail transcription, and E-cadherin is largely dependent on tissue and cell type.”

Introduction ¶3

PASSReviewer 2· 80% conf

The scientific foundation is well established with extensive citation of prior work on Slug, EMT, E-cadherin, and MMP-9, and a clear rationale linking Slug to MMP-9-driven invasion.

Evidence

“Recent work in hepatocellular carcinoma, breast cancer, and esophageal squamous cell carcinoma suggests that the transcriptional factors Snail and Slug are important effectors of the process of invasiveness and tumorigenicity through regulation of the expression of E-cadherin.”

Introduction ¶1

paraphrase[Introduction, paragraph 5]

“instead Slug enhanced cohort migration and Matrigel invasion by UMSCC1 cells, and additionally Slug increased MMP-9 levels”

“The difference may be because of the limited number of patients or the evaluation the immunohistochemistry results.”

Discussion ¶2

PASSReviewer 3· 80% conf

The introduction extensively cites prior work on Slug, EMT, E-cadherin, and MMP-9 and clearly frames the hypothesis that Slug may promote pancreatic cancer invasion via MMP-9 rather than E-cadherin.

Evidence

Introduction

“However, the relationship between Slug transcription, Snail transcription, and E-cadherin is largely dependent on tissue and cell type.”

Introduction

For the interventional components, the paper reports replicate distinction (duplicate experiments repeated four times) and includes appropriate controls (mock, vector, BB94 inhibitor). However, randomization of subjects/samples, blinding of investigators, a priori power analysis, and outlier handling are not reported. Inclusion/exclusion criteria for the clinical samples are described (consecutive cases with available tissue). Independent replication is not reported.

Sub-criteria

controlsADEQUATE

power analysisNOT REPORTED

blinding levelsNOT REPORTED

outlier handlingNOT REPORTED

randomization unitNOT REPORTED

inclusion exclusionADEQUATE

randomization methodNOT REPORTED

replicate distinctionADEQUATE

independent replicationNOT REPORTED

Per reviewer (3) — the votes behind the verdict

WARNReviewer 1· 85% conf

The study design has several strengths (replicates, controls) but lacks key rigor elements such as randomization, blinding, power analysis, and outlier handling.

Evidence

“Individual experiments were done in duplicate and repeated four times.”

Methods

paraphrase[Methods, BB94 Treatment and Cell Culture]

“PANC-1 cells were transfected with pcDNA3-Slug cDNA or control pcDNA3 vector; BB94 was used as a specific MMPs inhibitor.”

absence[Methods, all sections]

No randomization, blinding, power analysis, or outlier handling is reported.

WARNReviewer 2· 65% conf

Controls and patient inclusion criteria are adequately described, but randomization, blinding, power analysis, outlier handling, and independent replication are absent, with biological vs. technical replicates not clearly distinguished.

Evidence

“Slug-transfected or mock-transfected or untreated PANC-1 cells were plated at a density of 5 × 10 4 /mm 2 in culture dishes”

Methods

“Between 1999 and 2009, 60 consecutive pancreatic adenocarcinoma (ordinary ductal carcinoma) specimens for which tissue was available for immunohistochemical staining were identified”

Methods

“Individual experiments were done in duplicate and repeated four times.”

Methods

FAILReviewer 3· 75% conf

The study uses a descriptive clinical cohort plus cell-line and mouse experiments, but randomization, blinding, a priori power analysis, pre-specified inclusion/exclusion criteria, and independent replication are absent; only controls are adequately described.

Evidence

paraphrase[Materials and Methods, Patients and Tissue Samples]

“Between 1999 and 2009, 60 consecutive pancreatic adenocarcinoma (ordinary ductal adenocarcinoma) specimens for which tissue was available for immunohistochemical staining were identified from the files of General Surgery, the affiliated hospital of medical college, Qingdao University.”

“Individual experiments were done in duplicate and repeated four times.”

Methods

“The PANC-1 cells were transfected with 3 μ g of pcDNA3–Slug cDNA or control pcDNA3 vector as recently described using the Lipofectamine Plus reagent (Gibco BRL).”

Methods

For the animal study, sex (female), age (6-8 weeks), species/strain (C57BL/6), and source are reported, but housing conditions are only briefly mentioned ('sterile environment'). For the human cohort, no demographic details (age, sex, race/ethnicity) are provided. The use of only female mice is not justified. The cell line (PANC-1) is from ATCC, which is adequate.

Sub-criteria

demographicsNOT REPORTED

sex reportedNOT REPORTED

sex justifiedNOT REPORTED

age weight healthINADEQUATE

housing conditionsINADEQUATE

species strain sourceADEQUATE

Per reviewer (3) — the votes behind the verdict

FAILReviewer 1· 80% conf

Biological variables are inadequately reported: human patient demographics (age, sex) are absent, animal housing conditions are vague, and single-sex animal use is not justified.

Evidence

“Female C57BL/6 mice at 6–8 weeks old were obtained from Qingdao Medical college, Qingdao University”

Methods

absence[Methods, Patients and Tissue Samples]

Human patient demographics are not reported.

“All animals were maintained in a sterile environment and cared for within the laboratory animal regulations of the Ministry of Science and Technology of the People's Republic of China”

Methods

WARNReviewer 2· 60% conf

Mouse strain, age, and source are reported and PANC-1 is sourced from ATCC, but human-cohort demographics (age, sex) are absent, mouse sex is single-sex without justification, and housing conditions are only minimally described.

Evidence

“Female C57BL/6 mice at 6–8 weeks old were obtained from Qingdao Medical college, Qingdao University”

Methods

“The PANC-1 cell line used in present study was obtained from ATCC”

Methods

absence[Materials and Methods, Patients and Tissue Samples]

Human cohort demographics (age, sex) are not reported anywhere.

FAILReviewer 3· 75% conf

Species, strain, and source are reported for mice and PANC-1 cells, but human demographics (age, sex) are absent, the female-only mouse choice is not justified, and housing conditions are minimal.

Evidence

“Female C57BL/6 mice at 6–8 weeks old were obtained from Qingdao Medical college, Qingdao University for tumor implantation.”

Methods

paraphrase[Materials and Methods, Animal Studies]

“All animals were maintained in a sterile environment and cared for within the laboratory animal regulations of the Ministry of Science and Technology of the People's Republic of China.”

“The PANC-1 cell line used in present study was obtained from ATCC”

Methods

For human research, an ethics committee is named but no protocol number is given. The consent statement ('approved in written form by the patients studied') is ambiguous. For animal research, approval by the ethics committee is mentioned without a protocol number or specific IACUC name. Regulatory compliance with Chinese laboratory animal regulations is stated for animals, but no equivalent for human research (e.g., Declaration of Helsinki) is mentioned.

Sub-criteria

iacuc statementINADEQUATE

informed consentINADEQUATE

irb ethics statementINADEQUATE

regulatory complianceINADEQUATE

Per reviewer (3) — the votes behind the verdict

WARNReviewer 1· 85% conf

Ethics approval is mentioned but lacks protocol numbers, and consent description is vague. Regulatory compliance is partially addressed for animals but not for humans.

Evidence

“All research involving human participants was approved in written form by the patients studied and the ethics committee at the affiliated hospital of medical college, Qingdao University.”

Methods

“Full details of the study approval by the ethics committee at the affiliated hospital of medical college, Qingdao University.”

Methods

“All animals were maintained in a sterile environment and cared for within the laboratory animal regulations of the Ministry of Science and Technology of the People's Republic of China”

Methods

PASSReviewer 2· 60% conf

Ethics approval is stated for both the human cohort (named ethics committee, written consent) and the animal study (ethics committee approval plus named regulatory compliance), though without protocol numbers.

Evidence

“All research involving human participants was approved in written form by the patients studied and the ethics committee at the affiliated hospital of medical college, Qingdao University.”

Methods

“Full details of the study approval by the ethics committee at the affiliated hospital of medical college, Qingdao University.”

Methods

“cared for within the laboratory animal regulations of the Ministry of Science and Technology of the People's Republic of China”

Methods

WARNReviewer 3· 65% conf

Human research has a named ethics committee approval statement and written patient consent, and animal work cites national regulations, but the animal approval statement is vague and no protocol numbers are provided.

Evidence

“All research involving human participants was approved in written form by the patients studied and the ethics committee at the affiliated hospital of medical college, Qingdao University.”

Methods

“Full details of the study approval by the ethics committee at the affiliated hospital of medical college, Qingdao University.”

Methods

paraphrase[Materials and Methods, Animal Studies]

“All animals were maintained in a sterile environment and cared for within the laboratory animal regulations of the Ministry of Science and Technology of the People's Republic of China.”

Antibodies are identified by vendor and dilution (Santa Cruz Biotechnology, 1:100) but without catalog numbers, clones, or validation citations; the PANC-1 cell line is named with source (ATCC) but no STR authentication; mycoplasma testing is absent. Mice, primer sequences, plasmids, kits, inhibitors, and software (SPSS13.0, Quantity One) are adequately identified.

Sub-criteria

mycoplasma testingNOT REPORTED

reagents identifiedADEQUATE

organisms identifiedADEQUATE

antibodies identifiedINADEQUATE

cell line authenticationINADEQUATE

software tools identifiedADEQUATE

Per reviewer (3) — the votes behind the verdict

FAILReviewer 1· 90% conf

Key resources are inadequately identified: antibodies lack catalog numbers/RRIDs, cell line authentication and mycoplasma testing are absent, and reagents/software lack version and catalog details.

Evidence

“Slug (dilution at 1:100; Santa Cruz Biotechnology) or E-cadherin (dilution at 1:100; Santa Cruz Biotechnology), or MMP-9 (dilution at 1:100, Santa Cruz Biotechnology)”

Methods

“The PANC-1 cell line used in present study was obtained from ATCC”

Methods

“All statistical analyses were performed using SPSS13.0 software.”

Methods

WARNReviewer 2· 70% conf

Organisms, reagents, and software are identified but antibodies lack catalog numbers/RRIDs and validation references, PANC-1 is not authenticated, and mycoplasma testing is not reported.

Evidence

“Tissue slides were immunohistochemically stained for Slug (dilution at 1:100; Santa Cruz Biotechnology) or E-cadherin (dilution at 1:100; Santa Cruz Biotechnology), or MMP-9 (dilution at 1:100, Santa Cruz Biotechnology)”

Methods

“The PANC-1 cell line used in present study was obtained from ATCC”

Methods

“All statistical analyses were performed using SPSS13.0 software.”

Methods

WARNReviewer 3· 70% conf

Reagents, primers, and software are identified, and the PANC-1 source and mouse strain are given, but antibodies lack catalog/RRID/validation, and cell-line authentication and mycoplasma testing are not reported.

Evidence

Methods

“The PANC-1 cell line used in present study was obtained from ATCC”

Methods

“All statistical analyses were performed using SPSS13.0 software.”

Methods

The paper names the statistical tests used (ANOVA, Dunnett's t-test, Student's t-test, χ²). SPSS13.0 is identified. Data are presented as mean±s.d. with per-group n. However, test assumptions (normality, equal variance) are not verified. Effect sizes are not accompanied by confidence intervals. Many p-values are reported as '<0.05' or '<0.01' instead of exact values. No internal inconsistencies were detected in the reported numbers.

Sub-criteria

tests namedADEQUATE

exact p valuesINADEQUATE

effect sizes ciNOT REPORTED

data presentationADEQUATE

software identifiedADEQUATE

assumptions verifiedNOT REPORTED

mathematical plausibilityPASS

Per reviewer (3) — the votes behind the verdict

WARNReviewer 1· 80% conf

Statistical tests are named and software is identified, but assumptions are not verified, effect sizes without confidence intervals, and p-values are often reported as thresholds rather than exact values.

Evidence

paraphrase[Methods, Statistical Assessment]

“Differences between various groups were assessed using ANOVA or Dunnett's t-test.”

paraphrase[Results, Table 1]

“P <0.01”

absence[Results, throughout]

No confidence intervals for effect sizes are reported.

WARNReviewer 2· 65% conf

Tests and software are named and per-group data are shown, but p-values are mostly reported as thresholds, no effect sizes or confidence intervals are given, assumptions are not verified, and Table 2 contains an internal count inconsistency.

Evidence

“Differences between various groups were assessed using ANOVA or Dunnett's t -test. A P -value of <0.05 was considered to indicate statistical significance.”

Methods

“Slug, MMP-9, and E-cadherin expression was significantly associated with nodal metastases ( P <0.01, respectively”

Results

paraphrase[Table 2]

“Slug | Positive ( n =41) | Negative ( n =19) | | MMP-9 | Positive ( n =25) | 18 | 7 | | Negative ( n =35) | 17 | 18”

FAILReviewer 3· 75% conf

Statistical reporting is substantially incomplete: threshold p-values dominate, no effect sizes or confidence intervals are given, assumptions are not verified, and Table 2 contains an internal inconsistency in the stated group totals.

Evidence

“Differences between various groups were assessed using ANOVA or Dunnett's t -test.”

Methods

paraphrase[Results, The Relationship Between Slug, MMP-9, and E-Cadherin Expression and Pathological Data]

“Slug, MMP-9, and E-cadherin expression was significantly associated with nodal metastases ( P <0.01, respectively).”

“Positive ( n =41) | Negative ( n =19) | | | MMP-9 | | | 0.0034 | | Positive ( n =25) | 18 | 7 | | | Negative ( n =35) | 17 | 18”

Table 2

The paper does not include a data availability statement, nor does it deposit any data in a public repository or provide accession numbers. Custom code (if any) is not shared. This is a significant lack of transparency.

Sub-criteria

code sharingNOT REPORTED

accession numbersNOT REPORTED

repository depositNOT REPORTED

data availability statementNOT REPORTED

Per reviewer (3) — the votes behind the verdict

FAILReviewer 1· 95% conf

No data availability statement or data/code sharing is provided. The paper does not mention any repository deposit, accession numbers, or code repository.

Evidence

absence[Entire manuscript]

No data availability statement is present.

absence[Entire manuscript]

No repository deposit or accession numbers are mentioned.

absence[Entire manuscript]

No code sharing is mentioned.

FAILReviewer 2· 80% conf

No data availability statement, repository deposit, accession numbers, or code sharing are provided anywhere in the paper.

Evidence

absence[Entire manuscript]

No data availability statement exists in the paper.

absence[Entire manuscript]

No repository deposit or accession numbers are reported.

absence[Entire manuscript]

No code-sharing statement or repository is provided.

FAILReviewer 3· 90% conf

No data availability statement, repository deposit, accession numbers, or code-sharing mechanism is provided anywhere in the manuscript.

Evidence

absence[Entire manuscript]

No data availability statement is present.

absence[Entire manuscript]

No repository deposit or accession numbers are present.

absence[Entire manuscript]

No code-sharing statement or repository is present.

Methods are detailed but lack some elements (e.g., catalog numbers, blinding). All pre-specified outcomes appear to be reported, including negative results (no effect on E-cadherin). Conclusions are proportional to the evidence. However, no reporting checklist (e.g., ARRIVE) is referenced. The funding and conflicts of interest statement is incomplete ('The authors declare no'). Limitations are discussed implicitly but not in a dedicated section.

Sub-criteria

funding coiNOT REPORTED

trial registrationN/A

reporting guidelineNOT REPORTED

methods completenessINADEQUATE

all outcomes reportedADEQUATE

limitations discussedINADEQUATE

conclusions proportionalADEQUATE

Per reviewer (3) — the votes behind the verdict

WARNReviewer 1· 85% conf

The manuscript reports all outcomes and conclusions are proportional, but methods are incomplete, no reporting guideline is referenced, and funding/COI statements are missing/truncated.

Evidence

“no significant change was found in PANC-1 cells”

Results

“These findings indicate that Slug may regulate the metastatic nature of PANC-1 by stimulating cell motility”

Discussion

“The authors declare no”

End of manuscript

WARNReviewer 2· 65% conf

Methods are detailed and null results are reported, but no reporting guideline is cited, limitations are only briefly discussed, the funding/COI statement is truncated, and some conclusions overreach the data.

Evidence

“The authors declare no”

End of manuscript

“Slug may serve as a marker for the prediction of the malignancy of pancreatic cancer after operation.”

Discussion ¶2

“The present study for the first time has explored the relationship between Slug and MMP-9 in pancreatic cancer and clarified the novel mechanism.”

Discussion ¶5

WARNReviewer 3· 70% conf

Laboratory methods are detailed and negative results are reported, but no reporting guideline is referenced, funding is not stated, the limitations discussion is thin, and the conflict-of-interest statement is truncated.

Evidence

paraphrase[Results, Slug promotes MMP-9 Expression In vitro in PANC-1 Cells]

“We then analyzed the effect of Slug overexpression on E-cadherin expression in PANC-1 cells and no significant change was found in PANC-1 cells.”

“The difference may be because of the limited number of patients or the evaluation the immunohistochemistry results.”

Discussion

“The authors declare no”

End of manuscript

Broken references and links

None found · partly checked

References checked against Crossref, OpenAlex and Retraction Watch for retractions and resolvability, plus declared data and code links probed for whether they resolve to content matching the paper.

Nothing surfaced — but not everything feeding this category ran (missing: data/code link verification), so read this as a partial clean bill.

Checked 39 references by DOI: 29 verified — 10 no DOI (shown, not verified).

NO DOI
Transcriptional repressor Snail and progression of human hepatocellular carcinoma
No DOI in the reference — shown for manual review; not independently verifiable (not a fabrication signal).
NO DOI
Slug Expression in the E-cadherin preserved tumors is related to prognosis in patients with esophageal squamous cell carcinoma
No DOI in the reference — shown for manual review; not independently verifiable (not a fabrication signal).
NO DOI
Expression of SIP1 in oral squamous cell carcinomas: implications for E-cadherin expression and tumor progression
No DOI in the reference — shown for manual review; not independently verifiable (not a fabrication signal).
NO DOI
Expression of matrix metalloproteinase-9 in pancreatic ductal carcinoma is associated with tumor metastasis formation
No DOI in the reference — shown for manual review; not independently verifiable (not a fabrication signal).
NO DOI
Expression of matrix metalloproteinase-9 in surgically resected intrahepatic Cholangiocarcinoma
No DOI in the reference — shown for manual review; not independently verifiable (not a fabrication signal).
NO DOI
Expression of immunoreactive E-cadherin adhesion molecules in human cancers
No DOI in the reference — shown for manual review; not independently verifiable (not a fabrication signal).
NO DOI
Association of CC chemokine receptor 7 with lymph node metastasis of esophageal squamous cell carcinoma
No DOI in the reference — shown for manual review; not independently verifiable (not a fabrication signal).
NO DOI
Cutaneous wound healing: a partial and reversible EMT
No DOI in the reference — shown for manual review; not independently verifiable (not a fabrication signal).
NO DOI
The loss of E-cadherin, alpha- and beta-catenin expression is associated with metastasis and poor prognosis in invasive breast cancer
No DOI in the reference — shown for manual review; not independently verifiable (not a fabrication signal).
NO DOI
Relative expression of E-cadherin and type IV collagenase genes predicts disease outcome in patients with resectable pancreatic carcinoma
No DOI in the reference — shown for manual review; not independently verifiable (not a fabrication signal).

Copyediting

1 finding · worst low

Wording, consistency and formatting errors that need correcting before submission.

Wording or formatting errors that need correctingAssessed

19 copyedit issues flagged (11 major): mostly typo, clarity, consistency.

MAJORtypoTitle and abstract
“Slug enhances invasion ability of pancreatic cancer cells through upregulation of matrix metalloproteinase-9 and actin cytoskeleton remodeling Slug and invasion ability of pancreatic cancer cells”
→ Slug enhances invasion ability of pancreatic cancer cells through upregulation of matrix metalloproteinase-9 and actin cytoskeleton remodeling
Title is repeated; also missing punctuation.
MAJORtypoIntroduction
“F-ascin is an actin-bundling protein involved in filopodia assembly”
→ Fascin is an actin-bundling protein involved in filopodia assembly
Inconsistent spelling: 'F-ascin' vs 'F-actin' later.
MAJORgrammarEnd of manuscript
“The authors declare no”
→ The authors declare no conflicts of interest.
Sentence is incomplete; likely missing 'conflicts of interest'.
MAJORclarityMethods, Ethical statement
“All research involving human participants was approved in written form by the patients studied and the ethics committee”
→ All research involving human participants was approved by the ethics committee of the affiliated hospital of medical college, Qingdao University, and written informed consent was obtained from all patients.
Ambiguous phrasing; consent and approval are conflated.
MAJORclarityEnd of manuscript
“The authors declare no”
→ Complete the sentence, e.g., 'The authors declare no conflict of interest.' and add funding information.
Sentence is cut off mid-phrase.
MAJORconsistencyIntroduction, paragraph 1 and Discussion
“F-ascin is an actin-bundling protein involved in filopodia assembly”
→ Use 'F-actin' consistently throughout; 'F-ascin' appears to be a typo for 'F-actin'.
The protein is called F-actin elsewhere in the manuscript.
MAJORclarityFigure legends, opening fragment
“mined by immunohistochemistry. Staining of Slug was found in the cytoplasm”
→ Restore the missing beginning of the legend sentence, e.g., 'Expression of Slug was determined by immunohistochemistry.'
The sentence starts mid-phrase, indicating dropped text.
MAJORtypoMaterials and Methods, Cell Invasion Assays
“The chamers were put into the incubator”
→ The chambers were put into the incubator
Misspelling of 'chambers'.
MAJORclarityMaterials and Methods, Patients and Tissue Samples
“All research involving human participants was approved in written form by the patients studied and the ethics committee at the affiliated hospital of medical college, Qingdao University.”
→ The study was approved by the ethics committee of the affiliated hospital of medical college, Qingdao University, and written informed consent was obtained from all patients.
Consent and ethics approval are conflated in an awkward sentence.
MAJORconsistencyTable 3
“Table 3 Slug promoted in vivo tumor hepatic metastasis and lymph node invasion ( n =10) Groups ( n =12)”
→ Use n=12 consistently, or explain the discrepancy.
The caption says n=10 while the table header and text say n=12.
MAJORotherEnd of manuscript
“The authors declare no”
→ The authors declare no conflicts of interest. Funding: [state funding sources].
The sentence is truncated.
MINORconsistencyMethods, Immunohistochemistry
“Slug (dilution at 1:100; Santa Cruz Biotechnology) or E-cadherin (dilution at 1:100; Santa Cruz Biotechnology), or MMP-9 (dilution at 1:100, Santa Cruz Biotechnology)”
→ Slug (dilution at 1:100; Santa Cruz Biotechnology), E-cadherin (dilution at 1:100; Santa Cruz Biotechnology), or MMP-9 (dilution at 1:100; Santa Cruz Biotechnology)
Inconsistent use of semicolons and commas in list.
MINORtypoMaterials and Methods, Cell Invasion Assays
“The chamers were put into the incubator at 37°C”
→ Change 'chamers' to 'chambers'.
Spelling error.
MINORconsistencyTable 3 header
“Table 3 Slug promoted in vivo tumor hepatic metastasis and lymph node invasion ( n =10) Groups ( n =12)”
→ Clarify whether n=10 or n=12; the cell counts sum to 12 per group.
Header n=10 conflicts with the n=12 group sizes and cell sums.
MINORgrammarMaterials and Methods, Patients and Tissue Samples
“All research involving human participants was approved in written form by the patients studied and the ethics committee”
→ Rephrase to 'All research involving human participants was approved by the ethics committee... and written informed consent was obtained from the patients.'
Awkward and ambiguous phrasing of consent and approval.
MINORtypoAbstract / Introduction
“F-ascin”
→ Fascin
The protein name is misspelled as F-ascin.
MINORtypoMaterials and Methods, Western Blotting
“electrochemiluminescence kit (Amasham, Piscataway, NJ)”
→ electrochemiluminescence kit (Amersham, Piscataway, NJ)
Likely vendor name is Amersham.
MINORgrammarMaterials and Methods, Animal Studies
“Full details of the study approval by the ethics committee at the affiliated hospital of medical college, Qingdao University.”
→ The study was approved by the ethics committee of the affiliated hospital of medical college, Qingdao University.
This is a sentence fragment and does not clearly state that approval was obtained.
MINORclarityFigure 1 legend
“mined by immunohistochemistry.”
→ determined by immunohistochemistry.
Garbled phrase likely from a figure legend.

Kaimen Rigor Action ItemsFinal pass before journal submission

As a published work, the paper raises substantial robustness concerns. The internal inconsistency in Table 2 (cell counts not summing to stated group sizes) and the non-reproducible p-value for the Slug–MMP-9 association warrant a formal correction or independent re-analysis. The absence of data sharing, randomization, blinding, and cell line authentication further limit confidence in the reported findings. A reader should weigh these limitations heavily when interpreting the conclusions.

1.
CRITICALstatistics
Correct Table 2 so that the Slug-positive and Slug-negative column totals match the stated group sizes (n=41 and n=19), then recompute and report the exact p-value for the Slug–MMP-9 association using the corrected counts.
The current table contains an internal inconsistency that undermines the reported p-value and invalidates the statistical conclusions drawn from that correlation.
2.
CRITICALdata code
Add a data availability statement and deposit the raw datasets (IHC scores, qPCR, western blot densitometry, zymography, invasion counts, metastasis counts) in a public repository (e.g., Zenodo, Figshare, GEO) with a persistent identifier.
The paper provides no data sharing, which is a fundamental transparency requirement and prevents independent verification of the reported results.
3.
CRITICALreporting
Temper the claim that 'Slug may serve as a marker for the prediction of the malignancy of pancreatic cancer after operation' to a statement of association only, given the small correlative IHC series without prospective validation or multivariate adjustment.
This claim is not supported by the study design (single-cohort, 60 patients, no sensitivity/specificity analysis) and overstates the clinical utility of the finding.
4.
CRITICALreporting
Remove or qualify the novelty claim 'for the first time' regarding the relationship between Slug and MMP-9 in pancreatic cancer, as the paper provides no literature-search basis to substantiate priority.
Unsubstantiated priority claims are a common reviewer objection and can be perceived as misleading.
5.
HIGHrigor
Report randomization and blinding procedures for animal allocation and outcome assessment in the Animal Studies section, or justify an open design.
Absence of randomization and blinding is a major source of bias in interventional animal experiments.
6.
HIGHrigor
Add an a priori power analysis or sample-size justification for the clinical cohort, animal experiments, and cell assays in the Statistical Assessment section.
Without power analysis, the sample sizes are not justified, and the risk of false-negative or false-positive findings is unclear.
7.
HIGHother
Provide catalog numbers, clones, or RRIDs for all antibodies (Slug, E-cadherin, MMP-9) and cite validation references in the Immunohistochemistry and Western Blotting methods.
Antibodies without identifiers cannot be unambiguously identified by other researchers, compromising reproducibility.
8.
HIGHother
Report STR authentication and mycoplasma testing results for the PANC-1 cell line, or state that authenticated cells were used and mycoplasma testing was performed with negative results.
Cell line misidentification and mycoplasma contamination are common sources of irreproducible results.
9.
HIGHreporting
Complete the truncated conflicts-of-interest statement and add a funding statement with grant numbers in a dedicated section at the end of the manuscript.
The current statement is cut off mid-sentence, and funding information is entirely absent, which is a reporting requirement for most journals.
10.
HIGHreporting
Provide human cohort demographics (age, sex, tumor stage) and explicit inclusion/exclusion criteria in the Patients and Tissue Samples section.
Demographic details are essential for assessing generalizability and potential confounding in the clinical cohort.
11.
HIGHcopyedit
Correct the animal nomenclature inconsistency: clarify whether the mice were C57BL/6 or nude mice, and ensure consistent usage throughout the Methods and Results.
The Methods refer to 'Female C57BL/6 mice' but later mention 'nude mice'; this ambiguity could affect interpretation of the xenograft model.
12.
HIGHcopyedit
Correct the Table 3 caption: it states n=10 but the groups are labeled n=12 and cell counts sum to 12; change the caption to n=12 or explain the discrepancy.
This inconsistency confuses the reader and suggests a copyediting error that may affect data interpretation.
13.
MEDIUMstatistics
Replace all threshold p-values ('P<0.05', 'P<0.01') with exact p-values (2-3 significant figures) throughout the Results and Tables.
Threshold p-values obscure the exact evidence strength and are considered poor reporting practice.
14.
MEDIUMstatistics
Report effect sizes with 95% confidence intervals for all comparisons, and define error bars (SD or SEM) in figure legends.
Effect sizes and confidence intervals provide more informative measures of association than p-values alone.
15.
MEDIUMreporting
Cite an appropriate reporting guideline (ARRIVE for animal experiments, STROBE for the clinical cohort) in the Methods section.
Citing reporting guidelines improves transparency and helps ensure completeness of reporting.
16.
MEDIUMcopyedit
Fix the title: it is repeated in the manuscript; use 'Slug enhances invasion ability of pancreatic cancer cells through upregulation of matrix metalloproteinase-9 and actin cytoskeleton remodeling'.
The current title has a duplicated phrase ('Slug and invasion ability of pancreatic cancer cells'), which is a clear copyediting error.
17.
MEDIUMcopyedit
Correct 'F-ascin' to 'Fascin' throughout the Introduction and Discussion, and ensure consistent use of 'F-actin' where intended.
The misspelling 'F-ascin' appears to be a typo for 'Fascin' or 'F-actin', and consistency is needed for clarity.
18.
MEDIUMcopyedit
Rephrase the informed consent statement to clearly separate ethics approval from consent: 'The study was approved by the ethics committee... and written informed consent was obtained from all patients.'
The current phrasing conflates approval and consent and is grammatically ambiguous.
19.
MEDIUMcopyedit
Restore the missing beginning of the Figure 1 legend (currently starts with 'mined by immunohistochemistry'); likely should read 'Expression of Slug was determined by immunohistochemistry.'
The legend is truncated, which hinders understanding of the figure.
20.
MEDIUMcopyedit
Correct the typo 'chamers' to 'chambers' in the Cell Invasion Assays section.
Spelling errors reduce professionalism and clarity.
21.
LOWrigor
Add detailed housing conditions for mice (light cycle, temperature, humidity, enrichment) in the Animal Studies section.
Housing conditions can affect animal physiology and behavior, and their absence reduces reproducibility.
22.
LOWrigor
Provide a scientific justification for the use of only female mice, or include both sexes if feasible.
Single-sex studies without justification may limit generalizability and are increasingly scrutinized by journals.
23.
LOWother
Provide catalog numbers for all reagents (e.g., pcDNA3, Lipofectamine, G418, primers) and the specific version of the software (e.g., SPSS 13.0 for Windows).
Catalog numbers and version details facilitate precise replication of the methods.

Full text (39,745 chars)Analyzed: Aug 6, 2026

RigorReviewer 1

RigorReviewer 2

RigorReviewer 3

RigorPre-Submission Reviewer

CopyeditAgent Scribe

CitationsCitation Integrity

StatisticsStatistics Consistency

PreregistrationDeterministic check· no model (rule-based)

The star rating is the report’s one-glance summary. Every paper starts at 5★ and loses stars for the concrete problems the review finds — so a rating is never a vague average, it’s a running total you can read line by line under “How this rating was calculated.”

How stars are deducted

Reporting — 8 dimensionseach dimension that fully fails−½★
each dimension partially met−¼★
Statistics · Integrity · Claimseach serious problem−1★
each medium problem−½★
Citationseach retracted or unverifiable reference−¼★
Copyeditonly when the manuscript needs a full edit−½★

The rating never drops below 1★, and a demonstrable critical failure (an impossible statistic, a proven ethics violation) caps it at 1★ on its own — so the stars can never look healthy when the verdict is CRITICAL.

The rating draws on a panel of agents. Three independent Kaimen Rigor reviewers grade the eight dimensions below across several independent passes (the shown verdict is their majority vote — steadier than any single run), isolate the paper’s major claims and check its own evidence backs them, and flag integrity concerns. Alongside them, a citation agent resolves every reference against Crossref, OpenAlex, and Retraction Watch; a statistics agent recomputes reported tests; and rule-based checks verify that declared data/code links actually resolve. Full text is required — an abstract-only submission is not analyzed.

The eight reporting dimensions

Scientific PremisePrior work, rationale, and how it addresses gaps.

Study Design & RigorRandomization, blinding, power, controls, replication.

Biological VariablesSex, age, species/strain, housing, demographics.

Ethical ApprovalsIRB/IACUC approval, informed consent, regulatory compliance.

Key ResourcesAntibodies, cell lines, organisms, software — identified with persistent IDs.

Statistical ReportingNamed tests, exact p-values, effect sizes, plausible summary stats.

Data & Code AvailabilityAvailability statements, repositories, accession numbers, shared code.

Reporting TransparencyMethods, registration, guidelines, outcomes, limitations, funding/COI.

Graded against NIH, MDAR, ARRIVE 2.0, CONSORT, EQUATOR, and RRID guidelines. A dimension that doesn’t apply to the study type is skipped, never penalized.

This Kaimen Rigor review is model-assisted and is not a substitute for formal expert review. It complements human evaluation by surfacing potential methodological concerns — verify each finding against the source.

Slug enhances invasion ability of pancreatic cancer cells through upregulation of matrix metalloproteinase-9 and actin cytoskeleton remodeling

Zhang K, Chen D, Jiao X, Zhang S, Liu X, Cao J, Wu L, Wang D.

DOI

10.1038/labinvest.2010.201

Record issued

2026-08-06

Engine

7.15.0

Exported

2026-09-20