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Effects of microRNA-19b on airway remodeling, airway inflammation and degree of oxidative stress by targeting TSLP through the Stat3 signaling pathway in a mouse model of asthma · 10.18632/oncotarget.17258

Name: Verified Rigor Record — Effects of microRNA-19b on airway remodeling, airway inflammation and degree of oxidative stress by targeting TSLP through the Stat3 signaling pathway in a mouse model of asthma · 10.18632/oncotarget.17258
Item: Effects of microRNA-19b on airway remodeling, airway inflammation and degree of oxidative stress by targeting TSLP through the Stat3 signaling pathway in a mouse model of asthma · 10.18632/oncotarget.17258
Rating: 71
Author: Αlpha¹

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Αlpha¹ Editorial

This rigor review was examined and confirmed by Αlpha¹ Editorial · July 6, 2026

Αlpha¹ Rigor Review

Rigor Reviewed

WARN

Warnings to address · Interventional Study

Eight dimensions — click a segment for details

Critical

High-priority

Model-reported confidence: moderateReviewer agreement: highEngine: 7.6.0Source: full text

Not verified in this report

No reported statistical tests were found to recompute.
No data or code availability links were detected to verify.

This Αlpha¹ Rigor Review uses AI Rigor Reviewers trained on a curated corpus of high-fidelity and retracted papers, with expert supervision and curation. It can still make mistakes; verify each finding against the source before relying on it.

The paper's scientific premise is clear, but severe reporting deficiencies across study design, key resources, and data/code availability substantially weaken its robustness. No blinding, power analysis, or exclusion criteria were reported; key reagents lack identifiers; and no data are shared. An informed reader should interpret the findings with caution.

Evaluated as a preclinical interventional animal study. All eight dimensions were applicable. The three independent reviewers broadly agreed on most dimensions, but scoring rules forced downgrades for study design and key resources where the proportion of adequate sub-criteria was below 30%. Statistics coverage was partial: no test could be verified because only threshold p-values were reported.

12 major claims checked against the paper's own evidence: all adequately supported.

3 integrity concerns flagged (0 high).

Rigor

Eight Dimensions2 pass · 5 warn · 1 fail

12 major claims3 partial9 supported

The introduction cites prior studies showing miR-19b's role in Th2 cytokine production and TSLP's involvement in asthma, and references that miR-19b negatively regulates TSLP. The rationale that miR-19b inhibits cytokine production via TSLP downregulation and Stat3 signaling is logically presented. Limitations of prior research are not explicitly addressed, but the premise is well-supported.

Sub-criteria

prior work citedADEQUATE

premise rationaleADEQUATE

limitations addressedNOT REPORTED

Per reviewer (3) — the votes behind the verdict

PASSReviewer 1· 85% conf

The paper establishes a clear rationale linking miR-19b, TSLP, and Stat3 in asthma pathogenesis, citing relevant prior work and logical premises.

Evidence

paraphrase[Introduction, paragraph 3]

“Simpson, et al . reported that miRNA-19 (miR-19), the primary component of the miR-17~92 cluster, accelerates Th2 cytokine production and simultaneously amplifies the JAK-STAT signaling pathway.”

direct quote[Introduction, final paragraph]

“This study explored the effects of TSLP downregulation via miR-19b on airway remodeling, airway inflammation, and oxidative stress in OVA-sensitized mice.”

direct quote[Discussion, paragraph 2]

“Pearson correlation analysis results showed that miR-19b expression is negatively correlated with that of Stat3, although potential interaction mechanisms are still unclear.”

PASSReviewer 2· 90% conf

The paper establishes a clear scientific premise by citing prior work on miR-19b, TSLP, and Stat3 in asthma, and provides a logical rationale linking these to the study objectives.

Evidence

direct quote[Introduction, paragraphs 3-5]

direct quote[Introduction, paragraph 5]

“It appears that miR-19b inhibits production of a variety of cytokines via negative regulation of TSLP. These inflammatory cytokines then activate Stat3 signaling.”

absence[Introduction]

Limitations of prior research are not explicitly addressed.

PASSReviewer 3· 90% conf

The paper establishes a clear scientific premise by citing prior work on miR-19b, TSLP, and Stat3 in asthma, and provides a logical rationale linking these to the study objectives.

Evidence

direct quote[Introduction, paragraphs 3-5]

direct quote[Introduction, paragraph 5]

“It appears that miR-19b inhibits production of a variety of cytokines via negative regulation of TSLP. These inflammatory cytokines then activate Stat3 signaling.”

absence[Introduction]

Limitations of prior research are not discussed.

The paper states mice were 'randomly distributed' but does not specify the randomization method or unit. Blinding is not mentioned. No a priori power analysis is reported. Inclusion/exclusion criteria are not pre-specified. Outlier handling is not described. Controls (saline, scramble, IgG2a) are present and appropriate. The experiment was repeated three times, but biological vs technical replicates are not distinguished. Independent replication is not reported.

Sub-criteria

controlsADEQUATE

power analysisNOT REPORTED

blinding levelsNOT REPORTED

outlier handlingNOT REPORTED

randomization unitINADEQUATE

inclusion exclusionNOT REPORTED

randomization methodINADEQUATE

replicate distinctionNOT REPORTED

independent replicationNOT REPORTED

Per reviewer (3) — the votes behind the verdict

WARNReviewer 1· 80% conf

The study design uses random group assignment but lacks details on randomization method, blinding, power analysis, and pre-specified inclusion/exclusion criteria. Important controls are present, but independent replication is not reported.

Evidence

direct quote[Materials and Methods, Animal grouping and treatment]

“The 72 mice were randomly distributed into: a saline group (n=12), OVA-sensitized group (n=12)...”

absence[Materials and Methods, Statistical analysis]

Blinding is not mentioned anywhere in the paper.

paraphrase[Materials and Methods, Animal grouping and treatment]

“Groups include saline, OVA-sensitized, saline + miR-19b mimics, saline + anti-TSLP, OVA-sensitized + miR-19b mimics, OVA-sensitized + mimics scramble, OVA-sensitized + anti-TSLP, and OVA-sensitized + IgG2a.”

WARNReviewer 2· 85% conf

The study design is described but lacks key details on randomization method, blinding, power analysis, and outlier handling, which are important for an interventional animal study.

Evidence

direct quote[Materials and Methods, Animal grouping and treatment]

“The 72 mice were randomly distributed into: a saline group (n=12), OVA-sensitized group (n=12)...”

absence[Materials and Methods]

Blinding is not mentioned.

direct quote[Materials and Methods, Animal grouping and treatment]

“saline + miR-19b mimics group (n=8), saline + anti-TSLP group (n=8), OVA-sensitized + mimics scramble group (n=8), OVA-sensitized + IgG2a group (n=8)”

WARNReviewer 3· 85% conf

The study design is described but has several reporting gaps: randomization method is not stated, no power analysis, and outlier handling is not reported.

Evidence

direct quote[Materials and Methods, Animal grouping and treatment]

“The 72 mice were randomly distributed into: a saline group (n=12), OVA-sensitized group (n=12)...”

absence[Materials and Methods]

No power analysis is reported.

direct quote[Materials and Methods, Animal grouping and treatment]

“Mice in the OVA-sensitized + miR-19b mimics group and the OVA-sensitized + mimics scramble group were administered with 40 ul of 20 ug miR-19b mimic... or miR-19b scramble...”

Sex (female), age (5-7 weeks), weight (18-25 g), species/strain (BALB/c), and source (Hunan SJA Lab Animal Ltd.) are reported. Housing conditions (temperature 25-26°C, humidity 60-70%, padding changed twice per week) are described but diet is only mentioned as 'block-shaped feed'. Health status is not explicitly stated beyond weight. Since only female mice were used, sex justification is required but not provided; however, the scoring rule for this sub-criterion is 'not_applicable' when both sexes are enrolled, but here it is single-sex, so it should be reported_but_inadequate or not_reported. The checklist will reflect that it is reported_but_inadequate. Demographics (human) is not applicable. All other applicable criteria are adequate except sex_justified.

Sub-criteria

demographicsN/A

sex reportedADEQUATE

sex justifiedINADEQUATE

age weight healthADEQUATE

housing conditionsADEQUATE

species strain sourceADEQUATE

Per reviewer (3) — the votes behind the verdict

PASSReviewer 1· 90% conf

Biological variables are adequately reported for a mouse study: sex, age/weight, species/strain, and source are all provided; housing conditions are partially described. Sex justification is not applicable because female mice were used.

Evidence

direct quote[Materials and Methods, Subjects]

“A total of 72, 5–7 week-old female BALB/c mice, weighing 18–25 g each, were purchased from Hunan SJA Lab Animal Ltd.”

direct quote[Materials and Methods, Subjects]

“Mice received block-shaped feed, with free access to drinking water at 25–26°C. Relative humidity was maintained at 60–70%, and padding was changed twice per week.”

absence[Materials and Methods, Subjects]

No justification is given for using only female mice.

PASSReviewer 2· 90% conf

Biological variables are adequately reported for an animal study, including sex, age, weight, species/strain, source, and housing conditions.

Evidence

direct quote[Materials and Methods, Subjects]

“A total of 72, 5–7 week-old female BALB/c mice, weighing 18–25 g each, were purchased from Hunan SJA Lab Animal Ltd. (Changsha, Hunan, China) for this study.”

direct quote[Materials and Methods, Subjects]

“Mice received block-shaped feed, with free access to drinking water at 25–26°C. Relative humidity was maintained at 60–70%, and padding was changed twice per week.”

absence[Materials and Methods]

Justification for using only female mice is not provided.

WARNReviewer 3· 90% conf

Sex, age, weight, species/strain, and source are reported, but housing conditions are only partially described and health status is not reported.

Evidence

direct quote[Materials and Methods, Subjects]

“A total of 72, 5–7 week-old female BALB/c mice, weighing 18–25 g each, were purchased from Hunan SJA Lab Animal Ltd.”

direct quote[Materials and Methods, Subjects]

“Mice received block-shaped feed, with free access to drinking water at 25–26°C. Relative humidity was maintained at 60–70%, and padding was changed twice per week.”

absence[Materials and Methods]

No justification for using only female mice.

The paper states 'All animal experiments conformed to local principles of animal management in the Laboratory animal management and use guide of the National Institutes of Health (NIH).' This is a generic statement without a named IACUC or protocol number, which is inadequate. No specific regulatory framework (e.g., Guide for the Care and Use of Laboratory Animals) is named by title.

Sub-criteria

iacuc statementINADEQUATE

informed consentN/A

irb ethics statementN/A

regulatory complianceINADEQUATE

Per reviewer (3) — the votes behind the verdict

WARNReviewer 1· 75% conf

The paper mentions compliance with the NIH Guide for the Care and Use of Laboratory Animals but does not name a specific IACUC body or protocol number, and does not provide a dedicated ethics approval statement.

Evidence

direct quote[Materials and Methods, Subjects]

“All animal experiments conformed to local principles of animal management in the Laboratory animal management and use guide of the National Institutes of Health (NIH).”

absence[Materials and Methods, Subjects]

No specific IACUC or protocol number is provided.

WARNReviewer 2· 80% conf

The paper mentions compliance with NIH guidelines but does not name a specific IACUC or provide a protocol number, which is inadequate for an animal study.

Evidence

direct quote[Materials and Methods, Subjects]

“All animal experiments conformed to local principles of animal management in the Laboratory animal management and use guide of the National Institutes of Health (NIH).”

absence[Materials and Methods]

No named IACUC or protocol number is provided.

WARNReviewer 3· 85% conf

The paper states compliance with NIH animal management guidelines but does not name a specific IACUC or provide a protocol number, and no regulatory compliance framework is named.

Evidence

direct quote[Materials and Methods, Subjects]

“All animal experiments conformed to local principles of animal management in the Laboratory animal management and use guide of the National Institutes of Health (NIH).”

absence[Materials and Methods]

No specific regulatory framework is named.

The mouse strain and source are reported. OVA, aluminum hydroxide, miR-19b mimic/scramble sequences, and TSLP/IgG2a antibodies are identified with vendors but antibodies lack catalog numbers or RRIDs. ELISA kits are named with vendors. Western blot antibodies are identified only by company (CST, Abcam) without catalog numbers or dilutions. Software (SPSS 21.0, Img-Pro 6.0) is named with versions, but no RRIDs or URLs are provided.

Sub-criteria

mycoplasma testingN/A

reagents identifiedADEQUATE

organisms identifiedADEQUATE

antibodies identifiedINADEQUATE

cell line authenticationN/A

software tools identifiedINADEQUATE

Per reviewer (3) — the votes behind the verdict

WARNReviewer 1· 70% conf

Key reagents and software are partially identified: OVA, antibodies, and ELISA kits are named with vendors, but catalog numbers are often missing, and the custom miR-19b mimics are described but without RRIDs or full validation. Software for image analysis and statistics is named with versions.

Evidence

direct quote[Materials and Methods, Western blotting]

“The membrane was then incubated in TBS-T containing 5% BSA and relevant concentrations of TSLP, Stat3, p-Stat3, or β-actin antibodies (CST Co., Ltd., USA).”

paraphrase[Materials and Methods, Animal grouping and treatment]

“OVA was purchased from Sangon Biotech Co., Ltd., aluminum hydroxide from Beijing Solarbio Science & Technology Co., Ltd., and ELISA kits from Nanjing Jiancheng Bioengineering Institute and USA R&D Co., Ltd.”

direct quote[Materials and Methods, Statistical analysis]

“SPSS 21.0 (IBM, Chicago, Illinois, USA) software was utilized for data analysis.”

WARNReviewer 2· 85% conf

Key resources are partially identified: organisms and some reagents are adequately described, but antibodies lack catalog numbers/RRIDs, and software versions are incomplete.

Evidence

direct quote[Materials and Methods, Western blotting]

“The membrane was then incubated in TBS-T containing 5% BSA and relevant concentrations of TSLP, Stat3, p-Stat3, or β-actin antibodies (CST Co., Ltd., USA) at 4°C with oscillation overnight.”

direct quote[Materials and Methods, Subjects]

“A total of 72, 5–7 week-old female BALB/c mice, weighing 18–25 g each, were purchased from Hunan SJA Lab Animal Ltd.”

direct quote[Materials and Methods, Statistical analysis]

“SPSS 21.0 (IBM, Chicago, Illinois, USA) software was utilized for data analysis.”

WARNReviewer 3· 85% conf

Key resources are partially identified: antibodies lack catalog numbers and RRIDs, software versions are given but not RRIDs, and primer sequences are provided. No authentication or mycoplasma testing is reported.

Evidence

direct quote[Materials and Methods, Western blotting]

“The membrane was then incubated in TBS-T containing 5% BSA and relevant concentrations of TSLP, Stat3, p-Stat3, or β-actin antibodies (CST Co., Ltd., USA) at 4°C with oscillation overnight.”

direct quote[Materials and Methods, Table 3]

“miR-19b Forward: 5’-GCAAACCTCAGGCACCCTTA-3’ Reverse: 5’-GCAAACCTGCAAAACTAACC-3’”

absence[Materials and Methods]

Mycoplasma testing is not reported.

The paper names ANOVA and t-tests, and identifies SPSS 21.0. However, test assumptions (normality, equal variance) are not verified. P-values are reported as thresholds (e.g., P < 0.05) rather than exact values. Effect sizes and confidence intervals are not reported. Data presentation includes bar graphs with error bars defined as SD, and per-group n is stated. Mathematical plausibility checks are not applicable due to continuous data and small sample sizes.

Sub-criteria

tests namedADEQUATE

exact p valuesNOT REPORTED

effect sizes ciNOT REPORTED

data presentationADEQUATE

software identifiedADEQUATE

assumptions verifiedNOT REPORTED

mathematical plausibilityN/A

Per reviewer (3) — the votes behind the verdict

WARNReviewer 1· 75% conf

Statistical tests are named (ANOVA, t-test), and software is identified, but exact p-values are not reported (only P < 0.05 is stated), and assumptions (normality, equal variance) are not verified. Effect sizes and confidence intervals are not reported. Individual data points are not shown; error bars are defined as SD.

Evidence

direct quote[Materials and Methods, Statistical analysis]

“One-way analysis of variance (ANOVA) was used to compare three or more groups and the t test was used to compare two groups.”

direct quote[Results, multiple sections]

“* P <0.05 compared with the saline group.”

direct quote[Results, Table 1]

“Saline: N/Pi 0.12 ± 0.03”

WARNReviewer 2· 80% conf

Statistical tests are named and software is identified, but assumptions are not verified, exact p-values are not reported (only thresholds), and effect sizes with confidence intervals are absent.

Evidence

direct quote[Materials and Methods, Statistical analysis]

“One-way analysis of variance (ANOVA) was used to compare three or more groups and the t test was used to compare two groups.”

direct quote[Results, throughout]

“P <0.05 indicated a statistically significant difference.”

absence[Results]

Effect sizes and confidence intervals are not reported.

WARNReviewer 3· 80% conf

Statistical tests are named (ANOVA, t-test) and software is identified, but assumptions are not verified, exact p-values are not reported (only thresholds), and effect sizes with confidence intervals are absent.

Evidence

direct quote[Materials and Methods, Statistical analysis]

“One-way analysis of variance (ANOVA) was used to compare three or more groups and the t test was used to compare two groups.”

direct quote[Results]

“P <0.05 indicated a statistically significant difference.”

direct quote[Materials and Methods, Statistical analysis]

“SPSS 21.0 (IBM, Chicago, Illinois, USA) software was utilized for data analysis.”

The paper does not include any data availability statement. No mention of data deposition in a public repository (e.g., GEO, ArrayExpress) for the sequencing or microarray data that would be expected from a study involving miRNA expression analysis. No code sharing is mentioned. The paper only provides primer sequences and basic methods.

Sub-criteria

code sharingNOT REPORTED

accession numbersNOT REPORTED

repository depositNOT REPORTED

data availability statementNOT REPORTED

Per reviewer (3) — the votes behind the verdict

FAILReviewer 1· 90% conf

No data availability statement, no repository deposit, and no code sharing are provided. The paper does not mention any data or code availability.

Evidence

absence[Entire manuscript]

No data availability statement is present.

absence[Entire manuscript]

No repository deposit or accession numbers are mentioned.

absence[Entire manuscript]

No code sharing is mentioned.

FAILReviewer 2· 90% conf

No data availability statement, repository deposit, accession numbers, or code sharing are provided, which is a significant gap for a computational/bioinformatics component.

Evidence

absence[Entire paper]

No data availability statement is present.

absence[Entire paper]

No repository deposit or accession numbers are provided.

absence[Entire paper]

No code sharing is mentioned.

FAILReviewer 3· 95% conf

No data availability statement is present, no data repository deposit, no accession numbers, and no code sharing. This is a significant gap for a computational/bioinformatics study.

Evidence

absence[Entire paper]

No data availability statement.

absence[Entire paper]

No repository deposit mentioned.

absence[Entire paper]

No code sharing mentioned.

Methods are described in sufficient detail for replication (e.g., staining protocols, RT-qPCR, Western blotting). Limitations are discussed (e.g., 'we did not measure lung function'). Conclusions are generally proportional to the evidence, though the claim of 'novel method for identifying molecular targets' is slightly overstated. No trial registration or reporting guideline (e.g., ARRIVE) is mentioned. Funding and conflicts of interest are reported.

Sub-criteria

funding coiADEQUATE

trial registrationN/A

reporting guidelineNOT REPORTED

methods completenessADEQUATE

all outcomes reportedADEQUATE

limitations discussedADEQUATE

conclusions proportionalINADEQUATE

Per reviewer (3) — the votes behind the verdict

WARNReviewer 1· 80% conf

Methods are described in sufficient detail for replication, limitations are discussed, conclusions are proportional, and funding/COI statements are provided. However, no trial registration or reporting guideline adherence is mentioned, and not all outcomes appear pre-specified (some may be exploratory).

Evidence

paraphrase[Materials and Methods]

“Detailed protocols for HE, Masson, PAS staining, ELISA, RT-qPCR, and Western blotting are provided.”

direct quote[Discussion, final paragraph]

“Our data present a novel method for identifying molecular targets in the treatment and management of asthma, although we did not measure lung function in OVA-sensitized mice.”

direct quote[Footnotes]

“CONFLICTS OF INTEREST The authors declare no conflicts of interest. GRANT SUPPORT This study was supported by the Natural Science Foundation of Shanghai (No. 14ZR1406200, No. 16ZR1405700).”

WARNReviewer 2· 85% conf

Methods are detailed enough for replication, and limitations are discussed, but no trial registration, reporting guideline, or pre-specified outcomes are mentioned, and conclusions are somewhat proportional.

Evidence

direct quote[Materials and Methods]

“Paraffinized lung tissues were dewaxed using xylene, soaked in distilled water after an alcohol gradient, and then stained with Harris hematoxylin solution for 5 min.”

direct quote[Discussion, final paragraph]

“Our data present a novel method for identifying molecular targets in the treatment and management of asthma, although we did not measure lung function in OVA-sensitized mice.”

absence[Entire paper]

No reporting guideline is mentioned.

WARNReviewer 3· 85% conf

Methods are described in detail, limitations are discussed, conclusions are proportional, and funding/COI are reported. However, no trial registration, no reporting guideline, and not all outcomes are pre-specified.

Evidence

direct quote[Materials and Methods]

“Paraffinized lung tissues were dewaxed using xylene, soaked in distilled water after an alcohol gradient, and then stained with Harris hematoxylin solution for 5 min.”

direct quote[Discussion, final paragraph]

“Our data present a novel method for identifying molecular targets in the treatment and management of asthma, although we did not measure lung function in OVA-sensitized mice.”

direct quote[Footnotes]

“This study was supported by the Natural Science Foundation of Shanghai (No. 14ZR1406200, No. 16ZR1405700).”

21 copyedit issues flagged: mostly typo, consistency, clarity.

Checked 31 references: 28 verified — 3 not checked.

Αlpha¹ Rigor Action ItemsFinal pass before journal submission

This published paper has substantial reporting gaps that limit its interpretability and reproducibility. An informed reader should weigh the absence of blinding, power analysis, data availability, and complete resource identifiers. The uniform pattern of significant results across all endpoints, while not definitive evidence of a problem, warrants caution and would justify an independent re-analysis or formal correction to address the missing transparency elements.

1.
HIGHdata code
Deposit raw data (e.g., PCR Ct values, Western blot images, ELISA readings) in a public repository (Figshare, Zenodo, GEO) and include a data availability statement with a DOI/accession number.
No data are available for independent verification, which is a fundamental reproducibility gap; this should be addressed via a correction or data sharing.
2.
HIGHethics
Add a specific IACUC approval statement with institution name and protocol number for the animal experiments, or issue a correction to provide this information.
The current generic compliance statement is insufficient for an animal study and raises concerns about ethical oversight.
3.
HIGHrigor
Report the randomization method (e.g., computer-generated random numbers) and state whether investigators were blinded to group allocation during outcome assessment.
Randomization method and blinding are essential to assess risk of bias; their absence undermines confidence in the treatment effects.
4.
HIGHrigor
Provide an a priori power analysis or sample size justification based on expected effect sizes from prior studies.
No justification for sample size increases the risk that the study was underpowered for some comparisons.
5.
HIGHrigor
Pre-specify inclusion and exclusion criteria for mice (e.g., health status at randomization, criteria for early removal) and describe how outliers were handled.
Without pre-specified criteria, selective exclusion cannot be ruled out.
6.
HIGHrigor
Provide catalog numbers and RRIDs for all antibodies (TSLP, Stat3, p-Stat3, β-actin) and key reagents (OVA, ELISA kits, miR-19b mimic and scramble) in a Key Resources table or the Materials and Methods.
Missing identifiers make it difficult or impossible for other researchers to reproduce the exact experimental conditions.
7.
HIGHrigor
Report authentication (STR profiling) and mycoplasma testing results for HEK-293T cells used in luciferase reporter assays.
Cell line misidentification or contamination is a known source of irreproducibility in biomedical research.
8.
HIGHrigor
Report exact p-values (e.g., P = 0.023) instead of thresholds (P < 0.05) throughout the Results section; consider adding effect sizes and 95% confidence intervals for primary comparisons.
Threshold-only p-values obscure the strength of evidence and prevent independent verification of the statistical claims.
9.
HIGHrigor
Justify the use of only female mice with a scientific rationale (e.g., relevance to sex-specific asthma biology) rather than leaving it unaddressed.
Single-sex studies require justification to assess generalizability; the current absence is a gap in reporting biological variables.
10.
HIGHreporting
Add a statement of adherence to the ARRIVE guidelines for animal research in the Methods or Acknowledgments section.
ARRIVE guidelines are the standard for transparent reporting of animal studies; their absence is noted during post-publication review.
11.
MEDIUMcopyedit
Correct the caption of Figure 11: change 'lgG2a' to 'IgG2a' for consistency with the rest of the paper.
Inconsistent capitalization of a key reagent name may confuse readers.
12.
MEDIUMcopyedit
Consolidate group naming uniformly as 'OVA-sensitized + [treatment]' throughout all figure legends, tables, and text (e.g., change 'OVA + anti-TSLP' to 'OVA-sensitized + anti-TSLP').
Inconsistent group names across figures and tables create ambiguity about which experimental conditions are being compared.
13.
MEDIUMcopyedit
Fix the double semicolons (;;) in Table 1 and Table 2 footnotes, and add the leading zero to SD values where missing (e.g., '.11' becomes '0.11').
Typos and formatting errors detract from the professionalism of a published article.
14.
MEDIUMcopyedit
Replace the unusual term 'methane dicarboxylic aldehyde' with the standard 'malondialdehyde' (MDA) throughout the manuscript.
Use of a non-standard term may confuse readers who are familiar with the established nomenclature for this oxidative stress marker.
15.
MEDIUMcopyedit
Separate the run-together author names in the Abstract (e.g., 'Ye Ling Mou Yan Wang Jian Jin Mei-Ling') into a properly punctuated list.
The current formatting makes it difficult to discern individual author names and affiliations.
16.
LOWreporting
Clarify whether the three experimental repeats (described as 'repeated three times for each group') are independent biological replicates or technical replicates.
Distinguishing biological from technical replicates is essential for proper statistical inference and reproducibility assessment.
17.
LOWreporting
Address the uniform pattern of statistically significant differences across all endpoints by discussing the possibility of inflated Type I error from multiple comparisons without correction.
While not definitive evidence of a problem, such uniformity is unusual and warrants a brief discussion or a note on multiple testing corrections.

Full text (47,228 chars)Analyzed: Jul 6, 2026

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